Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. The 1kb dna step ladder has ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. Promega dna ladder molecular weight markers, 1kb dna ladder. How can i prepare working solution with 1 kb dna ladder. The 1,000bp band appears more intense than all other bands, which are of approximately equal intensity. Dna step ladders contain dna bands of defined sizes and equal intensity with fragment lengths at defined increments. Straightforward procedure for laboratory production of dna ladder. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis.
Standard dna polymerase assay conditions not pcr conditions. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double. The bands of the ladder each contain from 1 to 12 repeats of a 1018. Step ladders are not intended for concentration estimations. Promega 1kb dna step ladder molecular weight marker for electrophoresis 1kb dna step ladder to 10,000. Twofold serial dilutions of the ladder prepared in 1x blueorange loading dye.
Agarose gel image of 1kb dna ladder sizeselected using the pronex size selective. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Eleven fragments of1001,000bp in 100bp increments premixed with loading dye. Promega rna markers are suitable for size estimation of singlestranded rna from 0. The 500 bp band is easily distinguishable from the others due to its higher concentration. Our teams are in regular contact with suppliers and. View more information about our dna stains technology. Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. The dna fragments may be stained with ethidium bromide. I preferred this product because it has premixed dye thus its ready to use and lasts a.
For example, step ladder dna fragments could be in increments of 100bp from 100bp all the way up to 4000bp. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. All bands except the 5kb band are of equal intensity. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. Promega 1kb dna ladder molecular weight marker 1kb dna. For example, step ladder dna fragments could be in increments of 100bp from. The 1 kb and 3 kb bands contain more dna to provide internal orientation. The 500 bp band is easily distinguishable from the others due to. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye.
For optimal resolution, the recommended agarose gel concentration is 0. We dont know when or if this item will be back in stock. The 1 kb dna ladder is a unique combination of a number of. Source pcr products and doublestranded dna digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mm trishcl ph 8. Large dna size selection with the pronexr sizeselective. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The rna markers consist of a ladder of nine rna transcripts. Promega 1kb dna ladder molecular weight marker 1kb dna ladder.
Ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. Dna step ladders have defined sizes with exact incremental steps between bands. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. The resulting pcr product is visualized as a single band on an ethidium. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. Promega dna ladder molecular weight markers, 1kb dna ladder by fisher scientific. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. Identification of the preparationsubstance and of the company preparation name. The ladders are not intended for use in quantitative analysis. The doublestranded dna is digested to completion with. Quickload purple 100 bp dna ladder is stable for at least 6 months at 25c. The ladder is dephosphorylated and ready for 5 endlabeling with radioisotopes using t4 polynucleotide kinase, allowing visualization by autoradiography. The 500bp fragment is present at increased intensity for easy identification.
For the convenience, the amounts of the 500 and 1,000 bp. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. The resulting pcr product is visualized as a single band on an ethidium bromidestained agarose gel. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Quickload purple 1 kb dna ladder is stable for at least 6 months at 25c. The quickload purple 1 kb dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Genscripts pcr dna ladder is composed of seven bands of 100 bp, 300 bp, 500 bp, 700 bp, 1,000 bp, 1,500 bp, and 2,000 bp,respectively. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Generuler 1 kb dna ladder thermo fisher scientific. Each ladder is provided with a tube of 6x blueorange loading dye. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x.
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